Figure 2. Heat map of differential gene expression in the liver following three successive daily intravenous injections of exomeres or supmeres (300 μg).

Male C57BL/6 mice were injected via tail vein with exomeres or supermeres derived from DiFi cells. Mice were sacrificed 24 hours after the third injection. Liver tissue samples were immediately stored in RNAlater (Ambion) and RNA was isolated using an RNeasy kit (Qiagen). Details of RNA-seq library preparation and analysis can be be found in Methods (Zhang et al. 2021). Genes with a fold-change > 1.5 and a false discovery rate < 0.1 were considered to be differentially expressed (n = 3 in each group).