(A) cfDNA fragment size distribution for two representative cell free BAL (BAL cfDNA) samples. FU = Fluorescence units. BP = base pairs.
(B) DNA fragment size for BAL cfDNA stratified by a 500 bp threshold (orange > 500 bp, blue < 500 bp) for n = 12 patients (x-axis).
(C) Median sequencing depth obtained from BAL cfDNA samples with and without shearing.
(D) Number of mutations detected in both sheared and un-sheared samples or in sheared samples alone for the two patients from C. A total of 42 mutations were detected in sheared samples versus 18 in unsheared (p < 0.001).
(E) BAL DNA concentrations in 20 lung cancer patients for BAL cfDNA and BAL cellular DNA.
(F) Comparison of mean VAF% for BAL cfDNA and BAL cellular DNA for 15 patients. ND = not detected. P-value is displayed above the plot.