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. Author manuscript; available in PMC: 2022 Dec 30.
Published in final edited form as: Br J Pharmacol. 2022 Jul 24;179(20):4829–4843. doi: 10.1111/bph.15915

Figure 6: VC protocol identifies funny current as a target of quinine.

Figure 6:

A, Representative cell treated with quinine shows a reduction in inward current when the cell is clamped to a hyperpolarized potential of −120 mV, before 6000 ms. After 6000 ms, outward current is reduced as If reverses in direction before inactivating. B, the current trace during and around the segment meant to isolate If (shaded grey in panel A). C, Functional t-test shows a significant difference between quinine-treated cells and control cells throughout the period of the protocol when funny current would be active (C, top). The average difference between quinine-treated and control cells is between 0.8 and 1.2 pA/pF throughout this period (C, middle). The Kernik-Clancy iPSC-CM model funny current becomes active during the period when quinine-treated cells show a significant change in current and turns off just before the p value increases above .05 (C, bottom). D, Cells treated with quinine (labeled Qn, p=.0097) show a decrease in total current during the VC segment designed to isolate If.