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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: Cell Prolif. 2006 Aug;39(4):261–279. doi: 10.1111/j.1365-2184.2006.00388.x

Figure 3. FACS analysis of ERK dependency in mediating PMA-induced G2-phase arrest.

Figure 3

HeLa cells were synchronized at the G1/S boundary and released back into the cell cycle. Cells were left untreated (control), treated with PMA (0.1 µm) at 7 h after release, treated with U0126 (10 µm) at 6.5 h after G1/S release or treated with PMA and U0126 together. Cells were harvested at the times indicated after release from G1/S and the number of cells (Y axis) containing 2N or 4N DNA content (X axis) were determined by FACS.