Figure 2. Forced expression of Runx3 induces CD103 expression on CD4 T cells.

(A) Intracellular Runx3 expression in CD4 and CD8 LN T cells of WT and Runx3^Tg mice. Histograms show staining of anti-Runx3 (shaded) versus isotype control antibody (open). Data are representative (top), and bar graphs show a summary (bottom) of 3 independent experiments with a total of 3 WT and 7 Runx3^Tg mice (CD8) and 4 WT and 5 Runx3^Tg mice (CD4).

(B) Intracellular ThPOK expression in CD4 LN T cells of WT and Runx3^Tg mice. Histogram is representative (left) and the bar graph shows the summary (right) of 3 independent experiments with a total of 4 WT and 6 Runx3^Tg mice.

(C, D) Surface CD103 (C) and β7 (D) expression was assessed on CD4 and CD8 LN T cells of WT and Runx3^Tg mice. Histograms show staining of anti-CD103 or anti-β7 (shaded) versus isotype control antibody (open). Data are representative (left), and bar graphs show a summary of 3 independent experiments (right) with a total of 3 WT and 3 Runx3^Tg mice.

(E) Coexpression of CD103 and β7 in CD4 LN T cells of WT and Runx3^Tg mice. CD4 LN T cells were assessed for CD103 and β7, and the frequency of CD103, β7 coexpressing cells were determined. Data are representative (left), and bar graphs (right) show a summary of 2 independent experiments with a total of 3 WT and 3 Runx3^Tg mice.

(F) Phenotype of CD103⁺β7⁺ coexpressing CD4 LN T cells. Naïve and memory phenotype was assessed by CD44 versus CD62L staining on CD103⁺β7⁺ (bottom) and CD103-negative β7^lo (top) CD4 LN T cells. Data are representative of 2 independent experiments with a total of 3 WT and 3 Runx3^Tg mice.