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. Author manuscript; available in PMC: 2022 Sep 21.
Published in final edited form as: Front Ophthalmol (Lausanne). 2022 Apr 27;2:873486. doi: 10.3389/fopht.2022.873486

Figure 4. Effect of IL-1β on oxytocin-induced lacrimal gland MEC contraction.

Figure 4.

Lacrimal gland MEC were either left untreated (control) of incubated with IL-1β (10 ng/ml) for 2, 4, or 7 days. Cells were trypsinized for each time period, reseeded at low density and then stimulated with OXT (10−6 M) for 20 minutes. Changes in MEC size (i.e., contraction) following OXT stimulation was measured using ImageJ/Fiji software, as described in the Methods section. Chronic treatment of MEC with IL-1β significantly inhibited OXT-induced contraction at all three time points (Student’s t-test). Data are means ± SD; n=30-35 for day 2; n=28-29 for day 4 and n=34-35 for day 7 with all data from 3 independent experiments.