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. Author manuscript; available in PMC: 2023 Jan 10.
Published in final edited form as: Curr Protoc. 2022 Sep;2(9):e536. doi: 10.1002/cpz1.536

Figure 4.

Figure 4.

Lifting organotypic cultures to an air liquid interface. A,B) Carefully break the connection between the collagen plug and the side of the well by pressing a sterile lab spatula up and down around the edge. Do not run the spatula around the edge once pressed down. This can cause the cells on the plug to pull from the plug itself. C-D) Using the sterile lab spatula scoop underneath the collagen plug and place the collagen plug onto the metal grid with E-media underneath the collagen plug.