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. Author manuscript; available in PMC: 2023 Jan 10.
Published in final edited form as: Curr Protoc. 2022 Sep;2(9):e536. doi: 10.1002/cpz1.536

Figure 7.

Figure 7.

Measuring changes in pigment in organotypic skin cultures. Loss of keratinocytes desmoglein 1 results in an increase in pigment production and release from melanocytes through paracrine signaling. Fontana-Masson stain can be used to visualize pigment present in sections from organotypic cultures to demonstrate changes in pigment levels in different conditions. A) Representative images of organotypic culture sections stained with the Fontana-Masson stain. Organotypic cultures were created using either control NHEKs (shCTL) or desmoglein 1 knockdown keratinocytes (shDsg1). Scale bar = 20μm. B) Quantification of area positively stained with Fontana-Masson stain.