Figure 8.

BrdU incorporation and staining in organotypic cultures. Adding BrdU to the media of organotypic cultures allows the labeling of any cells that replicate their genome during the time BrdU is present. Staining for BrdU and for other cell markers to allow identification of cell types which transition through the cell cycle. A shows representative images of organotypic cultures, which were labeled for BrdU for 24 hours prior to harvesting and fixing the samples, and stained for BrdU, Plakoglobin to label cell borders, Melan A to label melanocytes, and DAPI. Basal keratinocytes label with BrdU at this timepoint, and can be used a positive control for BrdU staining. The image contains two melanocytes, one labeling positive for BrdU and one negative for BrdU. Scale bar = 10μm.