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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: FASEB J. 2022 Oct;36(10):e22514. doi: 10.1096/fj.202200765R

Figure 3: The effect of CNL treatment on formation of apoptotic- and autophagy-specific markers.

Figure 3:

A: MOLM-14 were treated with drugs alone or in combinations (CNL 15μM, Ara-C 1.25μM, venetoclax 50nM) for 24 hours, fixed and processed for active Caspase-3 staining. Percentage of active Cas-3 positive cells were accessed by flow cytometry; N=3, run in triplicates, ns-non-significant; #, p<0.005 vs. vehicle, shown are means +/− S.E.M.

B: MOLM-14 cells were treated with the drug combinations as indicated above, fixed and processed for Cytochrome C release assay. Percentage of cells from which Cytochrome C has been released were accessed by flow cytometry; N=3, run in triplicates, ns-non-significant; shown are means +/− S.E.M. #, p<0.001 vs. vehicle. C: MOLM-14 cells were treated with the drug combinations as indicated above, harvested and proteins extracted. Western blots were performed and quantitated via densitometry. LC3B-II/Actin numbers: relative LC3B-II autophagosome marker expression to vehicle normalized to actin loading. Representative Western of N=3 replicates.