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. Author manuscript; available in PMC: 2022 Oct 13.
Published in final edited form as: Sci Immunol. 2022 Oct 28;7(76):eabp9962. doi: 10.1126/sciimmunol.abp9962

Fig. 4. Structural analysis of SW186 Fab binding to RBD-SD1.

Fig. 4.

(A) N343-linked glycan binds to SW186 Fab through extensive hydrogen bonding. NAG: N-Acetylglucosamine, Fuc: Fucose. N343-linked glycan is colored green, and N343 is colored magenta (B) CDR3 region of the heavy chain partially inserts into the minor groove on the side of RBD. (C) CDR2 region interacts with RBD by polar interactions. The residues involved in Fab-RBD binding are shown as sticks. Dash lines represent hydrogen bonds. (D-H) Mutational analysis of SW186 by ELISA. Indicated mutants of SW186 were serially diluted and tested for their binding with S-ecto protein that was coated on an ELISA plate. Experiments were performed in duplicate. All data points are shown in plots. * indicates the significant difference between the indicated SW186 mutants and WT SW186 at 300 ng/mL as calculated by two-way ANOVA. p-values: ≤ 0.05 (*), ≤ 0.01 (**), ≤ 0.001 (***), ≤ 0.0001 (****).