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. Author manuscript; available in PMC: 2022 Oct 2.
Published in final edited form as: Cell Biol Toxicol. 2021 May 10;38(5):781–807. doi: 10.1007/s10565-021-09603-2

Fig. 2.

Fig. 2.

Influence of FRs on proliferative hNPCs (NPC1). Spheres were plated in 96-well U-bottom plates and exposed to increasing FRs concentration over 72 h. Proliferation was studied by measuring the increase of sphere area (NPC1a) (a) and by quantifying BrdU incorporation (NPC1b) (b) into the DNA. In parallel, viability and cytotoxicity (c) were assessed by performing Alamar Blue Assay and LDH Assay. Data are represented as means ± SEM (except EHDPHP in NPC1a and CTB n=2 mean ± SD). Highest concentrations (≥ 2.2 μM) of t-BPDPHP are not shown as spheres attached and differentiated. Statistical significance was calculated using one-way ANOVA followed by Bonferroni’s post hoc tests (p ≤ 0.05 was considered significant). BrdU, bromodeoxyuridine