Skip to main content
. Author manuscript; available in PMC: 2022 Dec 12.
Published in final edited form as: Cancer Immunol Res. 2022 Dec 2;10(12):1433–1440. doi: 10.1158/2326-6066.CIR-22-0418

Figure 2. Target antigen stimulation of CD19-targeted CAR T cells increases phosphorus-driven cellular metabolic demand.

Figure 2.

Basal, ATP-linked, maximal, and reserve oxygen consumption rate (OCR) and basal extracellular acidification were measured under basal metabolic conditions and in response to mitochondrial inhibitors, as specified in the methods after 24 hours of co-culture of (A) CD28 or (B) 4–1BB CAR T cells with CD19+ Raji target cells. (C) ATP production rate from oxidative phosphorylation and glycolysis normalized to cell density. Results are demonstrative of quintuplicate wells during independent experiment. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, unpaired t-test.