Figure 4. Rbms3 silencing cooperates with BRAF^V600E to promote the growth of lung organoids.

A: Representative images are shown of qualitative analyses of phase contrast images of organoids established following tumor dissociation from BC mice at 7 days post-initiation of organoids. White scale bar indicates 400 microns, and was taken with 10x magnification. N=12 technical replicates with 3 biological replicates leveraging pooled lung lobes from N=8 mice. B: Quantification of organoid diameters at 7 days post-initiation of organoids from lungs of the indicated mouse genotypes described in (A). C: qRT-PCR analysis of Rbms3 mRNA expression in organoids derived from BC mice labeled by the lentivirus they were initiated with. Transient over-expression of wild-type Rbms3 was used as a positive control for gene expression. Mean is graphed and error bars represent SEM. Statistical analysis was conducted using a paired T test; * = p < 0.05; **** = p < 0.0001.