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. Author manuscript; available in PMC: 2023 Mar 24.
Published in final edited form as: Cancer Res Commun. 2022 Dec;2(12):1657–1667. doi: 10.1158/2767-9764.CRC-22-0388

Figure 3. Six of ten dogs exhibited detectable anti-glioma humoral responses.

Figure 3.

Humoral responses to vaccination were quantified using flow cytometry-based endpoint titers with disaggregated J3T spheroids (A). Representative western blots (B/C) and confocal micrographs (D) depicting the serum antibody binding in a dog before (pre) immunization and 1-month post-vaccination (post). The western blots show binding to proteins found in the vaccine lysate (serum from dog 7) with the lower blot (C) depicting β-actin loading control. The confocal images tested binding of antibodies to J3T spheroids (D; serum from dog 3). All serum samples used in the assays were obtained after two vaccinations (week 4).