KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Biological samples | ||
|
| ||
| 3-dose HCWs Sera | Evans et al.1, Qu et al. 4 | N/A |
| Omicron BA.1-wave Infected Patient Sera | Evans et al.1, Qu et al. 4 | N/A |
| Omicron BA.4/5-wave Infected First Responders and Household Contacts Sera | This paper | N/A |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Polyethylenimine (PEI) | Thermo Fisher Scientific | Cat# BMS1003 |
| Dulbecco’s Modified Eagles Medium (DMEM) | Sigma-Aldrich | Cat#: 11965-092 |
| Fetal Bovine Serum (FBS) | Thermo Fisher Scientific | Cat#: F1051 |
| 0.05% Trypsin + 0.53 mM EDTA | Corning | Cat# 25-052-CI |
| Penicillin-Streptomycin | HyClone | Cat#: SV30010 |
| Immobilon Crescendo Western HRP substrate | Millipore | Cat# WBLUR0500 |
| QIAprep Spin Miniprep Kit | QIAGEN | Cat# 27106 |
| Coelenterazine | GoldBio | Cat#: CZ2.5, CAS: 55779-48-1 |
|
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| Deposited data | ||
|
| ||
| NT50 Values and De-identified patient data | SeroNet Coordinating Center, NCI, NIH | N/A |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| HEK293T | ATCC | Cat#: CRL-11268, RRID: CVCL_1926 |
| HEK293T-ACE2 | BEI Resources | Cat#: NR-52511, RRID: CVCL_A7UK |
|
| ||
| Calu-3 | ATCC | RRID: CVCL_0609 |
|
| ||
| Recombinant DNA | ||
|
| ||
| pNL4-3-inGluc | David Derse, NIH Maruzov et al. 47 |
N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_D614G | GenScript Biotech Zeng et al. 48 |
N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2 | GenScript Biotech Evans et al.1 |
N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5 | GenScript Biotech Qu et al. 4 |
N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4.6 | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BF.7 | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BQ.1 | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BQ.1.1 | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75 | GenScript Biotech Qu et al. 3 |
N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75.2 | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_K444T | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_N460K | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_N658S | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_R346T_K444T | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5_R346T_N460K | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R346T | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_F486S | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_D1199N | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R346T_F486S | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R346T_D1199N | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_F486S_D1199N | This paper | N/A |
|
| ||
| Software and algorithms | ||
|
| ||
| GraphPad Prism | GraphPad Version 9.0.0 | www.graphpad.com |
| SWISS-MODEL | Guex et al. 49 | https://swissmodel.expasy.org/ |
| PyMOL | Warren DeLano and Sarin Bromberg | https://pymol.org/ |
|
| ||
| Other | ||
|
| ||
| Cytation 5 Imaging Reader | BioTek | N/A |