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. Author manuscript; available in PMC: 2023 Jun 15.
Published in final edited form as: Nat Biotechnol. 2023 Jan 2;41(6):858–869. doi: 10.1038/s41587-022-01546-1

Fig. 3.

Fig. 3

Imaging of proteins, nucleic acids and lipids in biological specimens with Magnify.

a, Dotted bar chart showing lipid retention rate as a function of homogenization time in hot denaturing buffer. Error bars, s.e.m. b, Visualization of lipids in fully expanded Magnify-processed mouse brain. Top row, fully expanded mouse cortical neuron. Bottom row, zoom in of boxed region in top row. c, Visualization of a mitochondrion in Magnify-processed mouse brain with the lipophilic dye DiD. EF = 11×. d, Lipophilic dye staining of Golgi membranes in HEK-293FT cells expanded by Magnify. Right column, zoom ins of boxed area highlighting the Golgi body, e, Similar Magnify image of HEK-293FT cells as in d, but showing nuclear membrane labeling by DiD. EF = 9.22×. f, Lipophilic dye staining of blood vessels in mouse brain expanded by Magnify. EF = 10× in ddH2O. g, Electron micrograph of extracellular vesicles in human stem-cell-derived lung organoid, h, Two-color Magnify image of extracellular vesicles in the fully expanded human lung organoid with inverted look-up table. EF = 10.2×. Stain, green, Alexa Fluor 488 NHS ester; magenta, DiD. i, 3D reconstruction of h. j, Zoomed in view of boxed region in g. k, 3D reconstruction of the selected extracellular vesicle in h as indicated by the dashed blue box. l, Orthogonal view of the extracellular vesicle in k, showing complex internal structure. m, 3D reconstruction of confocal images of expanded human lymph node tissue labeled with DNA FISH probes against AKT1, Telomere (TelC) and human satellite 2. EF = 3.5× in 1× PBS. Gray, DAPI; green, AKT1; red, TelC; magenta, human satellite 2. n, 3D reconstruction of confocal images of expanded HEK-293FT cells. EF = 2.8× in 1× PBS. Blue, DAPI; green, RNA FISH probe against GAPDH; magenta, DNA FISH probe against human satellite 2. Scale bars (in biological scale), b, top, 5 μm, bottom, 2 μm; c, 250 nm; d,e, left, 5 μm, right, 1 μm; f, top, 5 μm, bottom, 2 μm; g,h, 3 μm; i,j, 1 μm; k, 500 nm. l, 200 nm; m, x, y and z: 7 μm; n, x, y and z: 10 μm.