Skip to main content
. Author manuscript; available in PMC: 2023 Jan 26.
Published in final edited form as: Alcohol Clin Exp Res (Hoboken). 2023 Jan 1;47(2):219–239. doi: 10.1111/acer.15000

Figure 3: Effect of chronic intermittent ethanol (CIE) exposure on the 5HT, ACE2, and TMPRSS2 immunoreactivity in dorsal raphe nucleus (DRN).

Figure 3:

Representative confocal images showing (A-B) 5-HT in the DRN (blue; scale bar = 100 μm) and (C-D) a single tile image (scale bar = 50 μm) from the boxed area of air control and CIE mice. (E-F) Representative images of ACE2 (red; scale bar = 100 μm) in the DRN and (G-H) a single tile image (scale bar = 50 μm) from the boxed area in air control and CIE mice. (I-J) TMPRSS2 (green; scale bar = 100 μm) in the DRN and (K-L) a single tile image (scale bar = 50 μm) from the boxed area in air control and CIE mice. (M-N) 5HT-ACE2 (magenta) colocalization in the DRN and (O-P) a single tile image from the boxed area in air control and CIE mice. (Q-R) 5HT-TMPRSS2 (white) positive neurons in the DRN and (S-T) a single tile image from the boxed area of air control and CIE mice. Histogram showing (U) % immunoreactive area for 5HT, ACE2, and TMPRSS2, (V) % immunoreactive area for 5HT-ACE2 positive neurons, (W) Pearson correlation coefficient for 5HT-ACE2 colocalization (X) Mander’s overlap coefficient for 5HT-ACE2 colocalization in DRN. Values (n = 4 - 5/group) are represented as means (±SEM) and the data was analyzed by unpaired student’s t-test with Welch’s correction (*p < 0.05 versus air control).