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. Author manuscript; available in PMC: 2023 Jan 26.
Published in final edited form as: Alcohol Clin Exp Res (Hoboken). 2023 Jan 1;47(2):219–239. doi: 10.1111/acer.15000

Figure 4: Effect of chronic intermittent ethanol (CIE) exposure on the TPH2 and ACE2 immunoreactivity in raphe magnus (RMG).

Figure 4:

Representative confocal images showing (A and D) TPH2 (green) (B and E) ACE2 (red) (C and F) TPH2-ACE2 (yellow) positive neurons in RMG of air and CIE mice. Scale bar = 50 μm. Graph showing (G) % immunoreactive area for ACE2 and TPH2, (H) % immunoreactive area for TPH2-ACE2 positive neurons, (I) Pearson correlation coefficient for TPH2-ACE2 colocalization (J) Mander’s overlap coefficient for TPH2-ACE2 colocalization in RMG. Values (n = 4 - 5/group) are represented as means (±SEM) and the data was analyzed by unpaired student’s t-test with Welch’s correction (*p < 0.05, **p < 0.01 versus air control).