Figure 3.

Perfusability of tumoral vessels in co-mixed and sequential tumor spheroids. a) Schematic showing dextran perfusion in tumor vessels. b) Representative confocal images of vascularized SN12C co-mixed or sequential tumor spheroids perfused with fluorescein dextran (10 kDa) on day 7. Device was imaged within 5 min after perfusing dextran. c) Statistical analysis of dextran percentage in the tumor region and tumor +50 μm region. Bars represent mean ± S.D. Two-tailed t tests were performed for the statistical comparisons. Data were collected from at least 6 tumor spheroids for each group. *p < 0.05.