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. Author manuscript; available in PMC: 2023 Feb 16.
Published in final edited form as: Circ Res. 2023 Jan 24;132(4):432–448. doi: 10.1161/CIRCRESAHA.122.322096

Figure 3. Effect of Mmp12 deficiency on aortic wall extracellular matrix composition.

Figure 3.

A. Representative examples of Verhoeff–van Gieson (VVG) staining of suprarenal abdominal aorta in Apoe−/− and Mmp12−/−/Apoe−/− (left column) and wild type (WT) and Mmp12−/− (right column) mice. Scale bar: 250 μm. B-G. Representative images of immunofluorescence staining and quantification of tropoelastin (red, panels B and C), collagen type I (red, panels D and E) and single stranded collagen using collagen hybridizing peptide, Cy3 conjugate (R-CHP) (red, panels F and G) in Apoe−/− and Mmp12−/−/Apoe−/− (panels B, D, and F) and WT and Mmp12−/− (panels C, E, and G) mice. Nuclei are stained blue with DAPI. n = 4 per group. Scale bar: 500 μm. H. Burst pressure, as a functional readout of wall strength, of descending thoracic aorta (DTA) and suprarenal abdominal aorta (SAA) in Apoe−/− and Mmp12−/−/Apoe−/− mice. n = 5 to 6 per group. *P < 0.05, **P < 0.01 by two-tailed Mann-Whitney U test (B-H).