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. Author manuscript; available in PMC: 2023 Feb 16.

Published in final edited form as: Circ Res. 2023 Jan 24;132(4):432–448. doi: 10.1161/CIRCRESAHA.122.322096

Figure 4. — A, B. Representative images of immunofluorescence staining and quantification of C3 deposits (red) in suprarenal abdominal aorta of Apoe^−/− and Mmp12^−/−/Apoe^−/− (A), and wild type (WT) and Mmp12^−/− (B) mice. Nuclei are stained with DAPI in blue. n = 5 per group. Scale bar: 500 μm. C, D. Plasma C3 and C5a levels of Apoe^−/− and Mmp12^−/−/Apoe^−/− (C), and WT and Mmp12^−/− (D) mice. n = 5 to 10 per group. E, F. Plasma C3 levels in Mmp12^−/−/Apoe^−/− (E) and Mmp12^−/− (F) mice at baseline and 9 days of treatment with IgG-FH_1–5 or MOPC antibody (control). n = 5 per group. G, H. Representative images of immunofluorescence staining and quantification of C3 (red) in suprarenal abdominal aorta of Mmp12^−/−/Apoe^−/− (G) and Mmp12^−/− (H) mice after 9 days of treatment with IgG-FH_1–5 or MOPC antibody (control). n = 5 per group; Scale bar: 500 μm. I, J. Representative images of immunofluorescence staining and quantification of Cit-H3 (red) in suprarenal abdominal aorta of Mmp12^−/−/Apoe^−/− (I) and Mmp12^−/− (J) mice after 9 days of treatment with IgG-FH_1–5 or MOPC antibody (control). n = 4 to 5 per group. Scale bar: 500 μm. *P < 0.05, **P < 0.01, ****P < 0.0001 by two-tailed Mann-Whitney U test (A-J).

Figure 4. — A, B. Representative images of immunofluorescence staining and quantification of C3 deposits (red) in suprarenal abdominal aorta of Apoe^−/− and Mmp12^−/−/Apoe^−/− (A), and wild type (WT) and Mmp12^−/− (B) mice. Nuclei are stained with DAPI in blue. n = 5 per group. Scale bar: 500 μm. C, D. Plasma C3 and C5a levels of Apoe^−/− and Mmp12^−/−/Apoe^−/− (C), and WT and Mmp12^−/− (D) mice. n = 5 to 10 per group. E, F. Plasma C3 levels in Mmp12^−/−/Apoe^−/− (E) and Mmp12^−/− (F) mice at baseline and 9 days of treatment with IgG-FH_1–5 or MOPC antibody (control). n = 5 per group. G, H. Representative images of immunofluorescence staining and quantification of C3 (red) in suprarenal abdominal aorta of Mmp12^−/−/Apoe^−/− (G) and Mmp12^−/− (H) mice after 9 days of treatment with IgG-FH_1–5 or MOPC antibody (control). n = 5 per group; Scale bar: 500 μm. I, J. Representative images of immunofluorescence staining and quantification of Cit-H3 (red) in suprarenal abdominal aorta of Mmp12^−/−/Apoe^−/− (I) and Mmp12^−/− (J) mice after 9 days of treatment with IgG-FH_1–5 or MOPC antibody (control). n = 4 to 5 per group. Scale bar: 500 μm. *P < 0.05, **P < 0.01, ****P < 0.0001 by two-tailed Mann-Whitney U test (A-J).