Table 7:

Comparison of normalized SARS-CoV-2 by PMMoV, B2M, and FIB for grab (n=24) versus composite samples produced in the laboratory from samples collected from the wastewater source each hour (Experiments 1-3B). p values of the one-sample T-test less than 0.05 indicate that the mean of the grab samples was statistically different than the value of the corresponding composite sample.

Normalized Molecular and Microbial Targets	SARS-CoV-2/PMMoV	SARS-CoV-2/B2M	SARS-CoV-2/FC
Sample Type	Experiment 1B - Building Scale
Grab Average (n=24)	4.24 × 10−4	5.12 × 10−1	1.57
Grab Median	3.26 × 10−5	5.75 × 10−3	8.28 × 10−4
Grab Range (min – max)	1.67×10−6–7.97×10−3	3.48×10−4–1.08×101	2.77×10−5–2.07×101
Grab Std. Dev.	1.62 × 10−3	2.21	4.85
Composite 1B	1.12 × 10−2	4.44 × 10−2	2.37 × 10−4
One-Sample T-Test (p-value: two-tailed)	0.353**	0.310**	0.178**
Sample Type	Experiment 2B - Cluster Scale
Grab Average (n=24)	2.10 × 10−3	2.85 × 10−1	9.88
Grab Median	1.48 × 10−3	1.70 × 10−1	1.55 × 10−2
Grab Range (min - max)	3.83×10−4–9.85×10f3	4.83×10−2–1.48	1.30×10−3–1.65×102
Grab Std. Dev.	2.15×10−3	3.15 × 10−1	3.61×101
Composite 2B	2.49 × 10−3	3.29 × 10−1	4.65 × 10−3
One-Sample T-Test (p-value: two-tailed)	0.384**	0.496**	0.009
Sample Type	Experiment 3B - Community Scale
Grab Average (n=24)	6.41 × 10−5	3.45 × 10−2	1.00 × 10−4
Grab Median	4.84 × 10−5	3.30 × 10−2	7.18 × 10−5
Grab Range (min – max)	9.32×10−6–3.33×10−4	7.09×10−3–1.15×10−1	1.11×10−5–5.02×−4
Grab Std. Dev.	6.32×10−5	2.13×10−2	9.90×10−5
Composite 3B	7.01 × 10−5	5.17 × 10−2	2.76 × 10−5
WWTP Composite	5.12 × 10−5	3.13 × 10−2	3.83 × 10−5
One-Sample T-Test (p-value: two-tailed) – Composite 3B	0.643**	<0.001	0.002
One-Sample T-Test (p-value: two-tailed) – WWTP Composite	0.328**	0.477**	0.005

^**denotes statistical equivalency between grab and composite sample types by One-Sample T-tests.