Figure 1. Detection of spontaneous lymphocyte proliferation in T, NK, and B cell populations.

(A) Cells were first gated to identify single cells that were Aqua-amine reactive dye negative (viable) and CD3+. Gated cells were plotted as CD4 vs. CD8 (left column). Gated CD4⁺ or CD8⁺ T cells were plotted vs. CFSE (middle and right columns, respectively), with proliferating cells falling in the CFSE^low gate. (B) Cells were gated to include viable single cells that were CD3⁻. Gated cells were plotted as CD16 vs. CD19 (left column). Gated CD16⁺ NK cells or CD19⁺ B cells were plotted vs. CFSE (middle and right columns, respectively), with proliferating cells falling in the CFSE^low gate. Representative data are shown for HTLV-1 infected (upper rows) and normal control subjects (lower rows).