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. Author manuscript; available in PMC: 2023 Feb 27.
Published in final edited form as: Traffic. 2022 Dec 15;24(2):52–75. doi: 10.1111/tra.12876

FIGURE 9.

FIGURE 9

Acute COG4 depletion displaces multiple vesicular coats from Golgi. (A) Airyscan superresolution IF analysis of untreated (control) or IAA treated) COG4-mAID cells stained for GM130 (green) and β′COP (red), (B) GM130 (green) and γCOP (red), (C) GM130 (green) and GGA2 (red), (D) GM130 (green) versus AP1-β (red), respectively. Scale bars, 20 μm. For the better presentation, green and red channels are shown in inverted black and white mode whereas the merged view is shown in RGB mode. (E) Colocalization of coat proteins with GM130 was determined by calculating Pearson’s correlation coefficient, >90 cells were analyzed. Statistical significance was calculated by GraphPad Prism 8 using paired t-test. Here, ***p ≤ 0.001 (significant) and ****p ≤ 0.0001 (significant). Error bar represents mean ± SD