Table 1.
Effect of acute COG4 depletion on Golgi protein | |||
---|---|---|---|
| |||
Early COG4 sensitive proteins | CCD vesicle protein | ||
(30–60 min depletion of COG4) | (2 h depletion of COG4) | COG4-insensitive Golgi proteins | |
COG subunits | COG1, COG3, COG8 |
||
SNAREs | GS15/BET1L, GS28/GOSR1 |
GS15/BET1L, GS28/GOSR1 |
STX5, YKT6 |
Coiled-coil tethers | Giantin/GOLGB1, TMF1/ARA160 |
Golgin84/GOLGA5, TMF1/ARA160 |
GM130/GOLGA2, P115/USO1 GOLGA1/Golgin97 GOLGA4/Golgin245 |
Small GTPases | Rab1B | Rab2A, Rab6A, Rab30A, Rab43A |
|
Coat proteins | β′COP/COPB2, γCOP/COPG1, GGA2, AP1β/AP1B1 |
||
Golgi enzymes | B4GalT1, | B4GalT1, | |
MGAT1 | MGAT1, GalNAcT2, Fut8 |
||
Other Golgi resident proteins | TMEM165/TPARL, GPP130/GOLIM4, TGN46/TGOLN2, Cab45/SDF4 |
GPP130/GOLIM4, Cab45/SDF4 |
ERGIC53/LMAN1 |
Cargo adaptor protein | GOLPH3 |