REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ADO (Rabbit polyclonal antibody)	OriGene	Cat#: TA322128; Lot#: D814AA091; RRID: AB_2920786
CBS (D8F2P) (Rabbit monoclonal antibody)	Cell Signaling Technology	Cat#: 14782; Lot#: 1; RRID: AB_2798609
CDO1 (Rabbit polyclonal antibody)	Proteintech	Cat#: 12589-1-AP; Lot#: 57877; RRID: AB_10638145
CSAD (Rabbit polyclonal antibody)	LSBio (LifeSpan)	Cat#: LS‑C375526; Lot#: 124060; RRID: AB_2801349
CSE (Rabbit polyclonal antibody)	Cell Signaling Technology	Cat#: 12217-1-AP; Lot#: 00089191; RRID: AB_2087497
FMO1 (Rabbit polyclonal antibody)	LSBio (LifeSpan)	Cat#: LS-C346135-50; Lot#: 215780; RRID: AB_2920787
GCLC (Mouse monoclonal antibody)	Santa Cruz Biotechnology	Cat#: sc-390811; Lot#: E1917; RRID: AB_2736837
GCLM (Rabbit polyclonal antibody)	GeneTex	Cat#: GTX114075; Lot#: 40156; RRID: AB_10619535
GSS (Mouse monoclonal antibody)	Novus	Cat#: NBP2-03351; Lot#: A01; RRID: AB_2920788
HSP90 (Rabbit polyclonal antibody)	Cell Signaling Technology	Cat#: 4874; Lot#: 6; RRID: AB_2233307
xCT (Rabbit polyclonal antibody)	Cell Signaling Technology	Cat#: 98051; Lot#: 1; RRID: AB_2800296
Chemicals, peptides, and recombinant proteins
Glucose	Fisher Scientific	Cat#: D16-500
Glycine	VWR	Cat#: BP381-1
L-Arginine·HCl	Sigma Aldrich	Cat#: A6969-25G
L-Aspartic acid	MP Biomedicals	Cat#: 194633
L-Asparagine	Sigma Aldrich	Cat#: A4159-25G
L-Cystine·2HCl	Sigma Aldrich	Cat#: C6727-25G
L-Glutamic acid	Sigma Aldrich	Cat#: G8415-100G
L-Glutamine	VWR	Cat#: 02-0131-0100
L-Histidine·HCl·H2O	Sigma Aldrich	Cat#: H5659-25G
Hydroxy-L-proline	TCI	Cat#: H0296
L-Isoleucine	Alfa Aesar	Cat#: J63045
L-Leucine	Sigma Aldrich	Cat#: L8912-25G
L-Lysine·HCl	Sigma Aldrich	Cat#: L8662-25G
L-Methionine	Sigma Aldrich	Cat#: M5308-25G
L-Phenylalanine	Sigma Aldrich	Cat#: P5482-25G
L-Proline	Sigma Aldrich	Cat#: P5607-25G
L-Serine	Sigma Aldrich	Cat#: S4311-25G
L-Threonine	VWR	Cat#: E808-25G
L-Tryptophan	Sigma Aldrich	Cat#: T8941-25G
L-Tyrosine·2Na·2H2O	Sigma Aldrich	Cat#: T1145-25G
L-Valine	Sigma Aldrich	Cat#: V0513-25G
[13C3]-serine	Cambridge Isotope Laboratories	Cat#: CLM-1574-H-0.1
[1-13C1]-serine	Cambridge Isotope Laboratories	Cat#: CLM-1573-0.25
[13C3]-cysteine	Cambridge Isotope Laboratories	Cat#: CLM-4320-H-PK
Methanol (LC-MS grade)	Thermo Scientific	Cat#: 047192.M1
H2O	Honeywell Burdick & Jackson™	Cat#: LC365-4
Acetonitrile	Honeywell Burdick & Jackson™	Cat#: 34998-2.5L
N-ethylmaleimide (NEM)	Alfa Aesar	Cat#: 40526-06
UltraPure™ Distilled Water	Invitrogen	Cat#: 10977-015
Ammonium formate	Frontier Scientific	Cat#: JK967458
DL-Propargylglycine	Fisher Scientific	Cat#: 437320010
Experimental models: Cell lines
A549	ATCC	Cat#: CCL-185; RRID: CVCL_0023
Calu3	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_0609
H1299	ATCC	Cat#: CRL-5803; RRID: CVCL_0600
H1581	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_1479
H1792	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_1495
H1944	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_1508
H1975	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_1511
H1993	ATCC	Cat#: CRL-5909; RRID: CVCL_1512
H2009	ATCC	Cat#: CRL-5911; RRID: CVCL_1514
H2172	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_1537
H2347	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_1550
H460	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_0459
HCC15	Dr. John Minna, Hamon Cancer Center Collection (University of Texas-Southwestern Medical Center)	RRID: CVCL_2057
DMS79	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1178
H211	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1529
H526	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1569
16HC	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_X025
16HV	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_X026
86M1	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_8263
H146	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1473
H1838	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1499
H209	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1525
H2107	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1527
H524	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1568
H740	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1586
H82	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1591
H841	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1595
HCC33	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_2058
N417	Moffitt Lung Cancer Center of Excellence Cell Line Bank	RRID: CVCL_1602
SW210.5	Dr. John Cleveland, Moffitt Cancer Center	RRID: CVCL_S185
PATU8902	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_1845
PL45	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_3567
MiaPaca2	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_0428
T3M4	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_4056
8988T	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_1847
8902	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_1845
BXPC3	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_5I88
CFPAC	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_1119
MPANC96	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_7165
PANC1	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_0480
SUIT2	Dr. Lewis C. Cantley, Weill Cornell Medicine	RRID: CVCL_3172
SW1990	ATCC	Cat# CRL-2172; RRID: CVCL_1723
Alex	Dr. Amaia Lujambio, Icahn School of Medicine	RRID: CVCL_0485
HepG2	Dr. Amaia Lujambio, Icahn School of Medicine	RRID: CVCL_0027
Hep3B	Dr. Amaia Lujambio, Icahn School of Medicine	RRID: CVCL_0326
SNU398	Dr. Amaia Lujambio, Icahn School of Medicine	RRID: CVCL_0077
SNU449	Dr. Amaia Lujambio, Icahn School of Medicine	RRID: CVCL_0454
Experimental models: Organisms/strains
LSL-KrasG12D (Krastm4Tyj)	JAX	RRID:IMSR_JAX:008179
Trp53flox (Trp53tm1Brn)	JAX	RRID:IMSR_JAX:008462
p48-Cre (Ptf1atm1(cre)Hnak)	Dr. Karen Mann, Moffitt Cancer Center	RRID:IMSR_JAX:023329
LSL-Nrf2D29H (Nfe2l2tm1Gmdn)	(28)	MGI:7327101
Rb1flox/flox; Trp53flox/flox; MycLSL/LSL (Igs2tm1(CAG-Myc*T58A/luc)Wrey Trp53tm1Brn Rb1tm3Tyj)	JAX	RRID:IMSR_JAX:029971
Recombinant DNA
pT3-EF1A-MYC-IRES-luc	(29)	RRID: Addgene_129775
CMV-SB13 vector	(29)
px330 p53	(29)
Software and algorithms
El-Maven	https://www.elucidata.io/el-maven	v 0.10.0 or 0.12.0
GraphPad Prism	https://www.graphpad.com/scientific-software/prism/	Version 9
IsoCor	https://isocor.readthedocs.io/en/latest/	Version 1.0 or 2.2.0
Xcalibur		Version 4.0
Other
RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)	US Biological	Cat#: R9010-01
Dulbecco’s MEM (DMEM) w/ L-Glutamine and Sodium Biscarbonate, w/o Glucose, Serine, Glycine (Powder)	US Biological	Cat#: D9800-16
Dialyzed FBS (dFBS)	Sigma Aldrich	Cat#: F0392
DMEM, 1× (Dulbecco’s Modification of Eagle’s Medium) with 4.5 g/L glucose, L-glutamine & sodium pyruvate	Corning	Cat#: 10-013-CV
RPMI Medium 1640 (1×) [+] L-Glutamine	Gibco	Cat#: 11875-093
DPBS w/o Calcium or Magnesium	Lonza	Cat#: 17-512F
FBS	Sigma Aldrich	Cat#: F0926