. Author manuscript; available in PMC: 2010 Apr 6.

Published in final edited form as: Microbiology (Reading). 2009 May 21;155(Pt 9):3055–3069. doi: 10.1099/mic.0.025031-0

Table 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant characteristics	Source or reference
E. coli strains
TOP 10	F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG	Invitrogen
S17-1	RP4 tra region, mobilizer strain recA derivative of MM294A with RP4-2 (Tc : : Mu : : Km : : FabF2/F17) integrated into the chromosome	Simon et al. (1983)
DH5α	F⁻ ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(r_k, $m_{k}^{+}$ ) phoA supE44 thi-1 gyrA96 relA1 λ⁻	Invitrogen
*R. leguminosarum* strains
3841	Biovar viciae, JB300 derivative, Sm^r	Johnston & Beringer (1975)
38EV27	3841 fabF2, fabF1 TGN mutant, Sm^r, Nm^r, Gm^r	This study
38EV51	3841 fabF2/F1 mutant with functional fabF1 expressed on plasmid pEV51, Sm^r, Nm^r, Gm^r, Tc^r	This study
38EV28	3841 fabF1 mutant, Sm^r, Gm^r	This study
Plasmids
pTGN	Tn5 derivative, Gm^r, Amp^r promoterless Nm^r, gfp	Tang et al. (1999)
pCR2.1 TOPO	TOPO TA cloning vector, 4.0 kb, Km^r, Ap^r	Invitrogen
pDG71	Broad-host-range derivative of pHC41. Constitutively expressed tryptophan promoter and gfp(mut3), Tc^r	Bringhurst et al. (2001)
pJQ200SK+	Suicide vector, P15aori, mob, sacB, Gm^r	Quandt & Hynes (1993)
pFUS1	pMP220 derivative with promoterless gusA, Tc^r	Reeve et al. (1999)
pFUS1P	pFUS1, par cassette inserted from pRR71-H as HindIII fragment	Yost et al. (2004)
pCS115	pWKR56 carrying HindIII fragment from pRIA76, Tc^r	Colonna-Romano et al. (1990)
pEV35	pCR2.1 TOPO with 1330 bp PCR fragment containing fabF1, Amp^r, Km^r	This study
pEV51	1330 bp fabF1 fragment cloned from pEV35 into pDG71 with BamHI, Tc^r	This study
pEV26	pCR2.1 TOPO with 456 bp PCR fragment containing internal fragment of fabF1, Amp^r, Km^r	This study
pEV28	456 bp fabF1 fragment cloned from pEV26 into pJQ200SK+ using ApaI and BamHI, Gm^r	This study
pNdvB	pFUS1P, ndvB : : gusA, Tc^r	This study
pAVP	pFUS1, flaA : : gusA, Tc^r	Michael Hynes
pVNVP	pFUS1P, visN : : gusA, Tc^r, par stabilized	Michael Hynes
pSVP SUM	pFUS1P, rem : : gusA, Tc^r, par stabilized	Michael Hynes
pCGR	pRK7813, mcpC : : gusA, Nm^r, Tc^r	Yost et al. (2004)
pDGRP	pFUS1P, mcpD : : gusA, Tc^r, par stabilized	Yost et al. (2004)