Table 2.
Sensitivity of the VLCFA mutants to detergents, desiccation and osmotic stress
| Strain | VMM+DOC* | VMM+SARC* | Desiccation tolerance† |
Hyperosmotic stress‡ |
Hypo-osmotic stress‡ |
|---|---|---|---|---|---|
| Wild-type | 125.0±4.45 | 123±3.42 | 42±1.8 | 79.4±1.42 | 57.0±8.08 |
| fabF2/F1 − | 12.9±4.72§ | 9.6±2.07§ | 3.46±1.68§ | 41.2±7.26§ | 10.7±0.970§ |
| fabF2/F1−+pCS115 | 110.0±7.67 | 110.2±7.73 | 15.7±5.42 | 90.7±4.57 | 50.8±2.72 |
| fabF2 − | 16.1±9.75§ | 17.6±2.56§ | nd | 30.1±5.21§ | 26.2±2.79§ |
| fabF1 − | 10.5±0.502§ | 10.9±1.05§ | nd | 47.1±2.08§ | nd |
| fabF1−+pCS115 | 94.8±4.64 | 74.5±6.82 | nd | 87.9±6.57 | nd |
Strains were grown in VMM broth or VMM supplemented with 0.075 % DOC or 0.050 % sarcosyl (SARC) for 2 days. OD600 values were measured. Percentage growth was calculated as described in Methods. Values are the mean (±sd) from three independent trials.
Desiccation sensitivity assays were performed as described in Methods. Percentage survival values presented are the mean (±sd) percentage survival from three independent trials.
Strains were grown in TY supplemented with 69.5 mM NaCl (hyperosmotic) or GYM medium (hypo-osmotic) for 2 days, and the growth was compared with that of strains grown in TY medium. OD600 values were measured. Percentage growth values are the mean and sd from three independent trials.
Difference between the wild-type and mutant is statistically significant at P <0.001 (Student’s t test).