Fig. 5.

The Hsd17b12^114T and Hsd17b12_114–122 peptides have similar affinities for H2-K^d molecules, but only the Hsd17b12^114T peptide is recognized by the Meth A-specific CTL. a Binding of the peptides to T2-K^d cells, as defined by mean fluorescence intensity (MFI) of peptide-pulsed cells maintained at 37°C in a MHC stabilization assay using anti-H2-K^d mAb and FITC-conjugated anti-mouse IgG antibody. The MFI of T2-K^d cells alone (asterisk) or pulsed with peptides (plus) and maintained at 4°C are shown. b Cytolytic reactivity of Meth A-specific CTL against T2-K^d cells pulsed with peptides at E/T ratio of 6:1 in 4 h ⁵¹Cr-release assays. The Meth A mutant p53_232–240 peptide (KYICNSSCM) was used as a negative control peptide