Figure 1. Single-cell RNA-seq of fate-mapped CD45⁺ cells derived from CX3CR1⁺ precursors during Schistosoma mansoni infection reveals BIRC5⁺ immune cells.

(A) Experimental design for single-cell RNA-seq of CD45⁺ from Cx3cr1^{CreERT2-EYFP/+} R26^tdTomato/+ mice infected cutaneously with 75 S. mansoni and sorted 8 weeks post-infection. Gating was on singlets, live cells, CD45⁺, tdTomato⁺. (B) UMAP representation and unbiased clustering of major immune cell populations from the CD45⁺ tdTomato⁺ cells sequenced, with each dot representing individual cells. (C) Expression of canonical cellular markers for the major clusters of immune cells. (D) Heatmap showing the markers of the main populations of immune cell clusters (E) Identification of BIRC5⁺ proliferative cluster.