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. Author manuscript; available in PMC: 2023 Sep 14.
Published in final edited form as: Nat Biotechnol. 2023 Jan 26;41(9):1272–1286. doi: 10.1038/s41587-022-01648-w

Extended Data Fig. 9 |. USeqFISH validation in NHP tissue.

Extended Data Fig. 9 |

a, Virally labeled cells, expressing mTurquoise2 (pseudo-colored), in the rhesus macaque brain and USeqFISH labeling with probes targeting the sequence of mTurquoise2. b, We validated our probe design for the rhesus macaque by applying USeqFISH with Pvalb probes and post hoc IHC with Pvalb antibodies to the same tissue. Localization of Pvalb mRNA signal in cells labeled with Pvalb antibodies (the same approach used for mouse probe validation in Supplementary Fig. 3) supports the versatility of our probe design in both rodents and NHPs.