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. Author manuscript; available in PMC: 2010 Sep 30.
Published in final edited form as: J Neurosci. 2010 Mar 31;30(13):4757–4766. doi: 10.1523/JNEUROSCI.6108-09.2010

Figure 6. The mobility of GFP-SAP102 is rapid regulated by synaptic activity.

Figure 6

A. The images represent FRAP of a neuron expressing GFP-SAP102 with blockade of NMDA and AMPA receptors. Neurons were incubated with 100 μM DL-APV and 20 μM DNQX 1 hour before experiments and during experiments. Scale bar, 2 μm. B–C. FRAP of GFP-SAP102 was reduced by blocking both NMDA and AMPA receptors activity. The immobile and slow mobile fractions of GFP-SAP102 decreased after APV and DNQX treatment. There was no change in rapid mobile fractions after APV and DNQX treatment. N = 4 for each FRAP curve, *p<0.05, Ctrl = control (black), A+D = APV+DNQX (gray). D. The images represent FRAP of a neuron expressing GFP-PSD-95 with blockade of NMDA and AMPA receptors. Scale bar, 1 μm. E–F. FRAP of GFP-PSD-95 was not affected by APV and DNQX treatment. There was no change in immobile, slow mobile and rapid mobile fractions after APV and DNQX treatment. N = 4 for each FRAP curve.