A. The images represent FRAP of a neuron expressing GFP-SAP102 with blockade of NMDA and AMPA receptors. Neurons were incubated with 100 μM DL-APV and 20 μM DNQX 1 hour before experiments and during experiments. Scale bar, 2 μm. B–C. FRAP of GFP-SAP102 was reduced by blocking both NMDA and AMPA receptors activity. The immobile and slow mobile fractions of GFP-SAP102 decreased after APV and DNQX treatment. There was no change in rapid mobile fractions after APV and DNQX treatment. N = 4 for each FRAP curve, *p<0.05, Ctrl = control (black), A+D = APV+DNQX (gray). D. The images represent FRAP of a neuron expressing GFP-PSD-95 with blockade of NMDA and AMPA receptors. Scale bar, 1 μm. E–F. FRAP of GFP-PSD-95 was not affected by APV and DNQX treatment. There was no change in immobile, slow mobile and rapid mobile fractions after APV and DNQX treatment. N = 4 for each FRAP curve.