Fig. 4. Evaluating guide-pooled Perturb-seq versus conventional Perturb-seq.

(a) Number of channels and droplets from the conventional validation screen (top) and guide-pooled screen (bottom). We focus our analysis on the subset of 8,448 droplets from the guide-pooled screen with at least 3 guides per droplet. (b) Distribution of cells based on # of guides they contain for the full guide-pooled and conventional screens. In practice, we only directly measure the # of guides/droplet rather than guides/cell, but these quantities are equivalent given 1 cell/droplet. (c-f) See captions for Fig. 3c–f. These analyses were conducted in an identical fashion, with the only difference that the screens are down-sampled based on cell count rather than channel count.