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. Author manuscript; available in PMC: 2023 Sep 26.
Published in final edited form as: Immunohorizons. 2023 Jan 1;7(1):41–48. doi: 10.4049/immunohorizons.2200097

Figure 1. Ornithine decarboxylase (ODC) is upregulated in IL-23-activated ILC3s.

Figure 1

(A) MNK-3 cells were treated with IL-23 for 4 hours or left untreated and analyzed by NanoString nCounter analysis using a mouse metabolism panel with 748 genes and 20 internal reference genes. Shown is a volcano plot with the Odc1 marked. (B) MNK-3 cells were treated with IL-23 or IL-1β for 4 hours or left untreated (NT). Il22 and Odc1 mRNA levels were semi-quantitated by real-time RT-PCR. Each point represents one well, bar indicates mean, n=6. Data are representative of three independent experiments. (C) MNK-3 cells were treated with IL-23 or IL-1β for 18 hours or left untreated (NT) and cell lysates were analyzed by western blotting to examine ODC and actin levels. Each point represents one well, line indicates mean, n=3. Data are representative of two independent experiments. p<0.0001 (****). Differences that were not significant (p>0.05) are marked as ns.