Fig 3.

Both AMPAR amplitude and single-channel conductance was reversed by intracellular inclusion of a CaMKII inhibitor. As recorded in ACSF alone (Shen et al., 2009) and as shown in Table 1, mEPSC (A) amplitude and (C) conductance were significantly increased in 2-day FZP-withdrawn rats in the negative control condition, with scrambled autocamtide inhibitory peptide (sAIP) in the whole-cell micropipette. (B) Representative plot of the results of NSNA of mEPSCs showing an increased current amplitude and slope conductance in an FZP-withdrawn neuron with sAIP (open circles/dotted line) versus a CON neuron with sAIP (close circles/black solid line). Intracellular inclusion of the active CaMKII inhibitor, +AIP reversed both the increased AMPAR-mediated mEPSC (A) amplitude and (C) elevated single-channel conductance to control levels (+sAIP) as illustrated in (B) (grey circles/grey solid line). CaMKIINtide inclusion in a few neurons had a similar effect, suggesting that AMPAR potentiation is mediated by CaMKIIα activation.