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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Neuropsychopharmacology. 2010 May 5;35(9):1897–1909. doi: 10.1038/npp.2010.61

Fig 5.

Fig 5

GluA subunit levels in CA1 PSD-enriched subcellular fractions as a function of time after drug withdrawal. PSD-enriched subfractions were collected by ultracentrifugation of TritonX-100-resistant membranes pooled from 3 hippocampal CA1 minislices as described in the methods. Representative immunoblots of total GluA1 and pSer831GluA1 with their respective loading controls are shown in PSD-enriched subfractions from (A) 1-day and (B) 2-day FZP-withdrawn rats in the leftmost panels. Histograms of integrated signal density as a percent of paired control density (n=4-11 lanes/group) are shown to the right. Only total-GluA1 expression levels were significantly enhanced in 1-day FZP withdrawn rats, while both total and pSer831GluA1 levels were increased in 2-day FZP withdrawn rats. There were no changes in GluA2 levels in 1-day (or 2-day, Das et al, 2008; Song et al, 2007) FZP-withdrawn rats. There were also no changes in GluA3 expression levels in the PSD-enriched fraction from 2-day FZP withdrawn rats. Asterisks denote p<.05.