Skip to main content
. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: J Immunol. 2023 Dec 15;211(12):1814–1822. doi: 10.4049/jimmunol.2300607

Figure 1. Generation of endogenous CD40 cytoplasmic domain mutant mice.

Figure 1.

A. Schematic representation of WT CD40 and mutants. CD40ΔT6 was generated by deletion of the sequence 234RQDPQE239. CD40ΔT2 was made by deletion of the sequence 251PVQET255. CD40ΔT2* was made by truncation of sequence distal to amino acid 245 by inserting two stop codons (TAATAG). B. Representative FACS analysis of CD40 surface expression on the same mouse spleen DCs (PI−CD11c+B220−) and B cells (PI−CD11c−B220+) that were in 4°C overnight (gray) or incubated with CpG overnight (red). C. CD40 was immunoprecipitated from cell lysates of the indicated mice and analyzed bound proteins immunoblotting by using TRAF2 antibody. Lower two panels showed expresion of TRAF2 and CD40 in whole cell lysates. The arrow indicates the position of TRAF2 and the asterisk indicates the position of a non-specific band present even in CD40−/− mice (*ns).