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. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: J Immunol. 2023 Dec 15;211(12):1814–1822. doi: 10.4049/jimmunol.2300607

Figure 3. Both CD40 TRAF6 and TRAF2/3 binding motifs function in EAE by regulating Th17 cell priming.

Figure 3.

WT or CD40 mutant mice were immunized with rhMOG and 6 days after EAE induction CD4+ T cells from the DLN of the indicated mice were analyzed by FACS analysis. A. DLN cells from rhMOG-immunized CD40fl/fl mice, CD40−/− mice, or CD40fl/fl mice crossed with CD19-Cre or CD11c-Cre (to delete CD40 only on B cells or DCs, respectively) were analyzed for cytokine production by FACS analysis (in the CD4+ B220− gate) and the percentage of CD4 T cells expressing IL-17A was determined. B. DLN CD4+ T cells from rhMOG-immunized WT or CD40 TRAF binding-motif mutant mice were analyzed for cytokine production by FACS analysis (in the CD4+ B220− gate). The number of CD4 T cells in the DLN (C) and the percentage of all DLN CD4 T cells expressing either IL-17A or IFN- g was determined (D). Data are combined from two independent experiments (mean ± S.D. using 6 mice per group). One-way ANOVA followed by Dunnett’s test against the immunized WT group was performed for multiple comparisons. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.