Table 2. Example data reports generated by CyteSeer®.
Cell by cell readout: LDC, Area Lm, the Ratio of Area Lm/Area Wm, Tii Li Lm, Api Li Lm, and Mpi Li and Lm are reported for an experiment with AML12 cells. Data is shown for the first 6 cells from well A1, out of a total of 914 cells that were imaged and analyzed for this well. Area Lm is in pixel units. Well by well readout: The number of cells (NOC) analyzed per well is shown, along with the mean values of the data parameters (averaged across all cells that were imaged in the well) for the first 6 wells in this experiment (out of a total of 96 wells). Additional population statistics can also be reported for each data parameter including medians and standard deviations. A1 is a control well, A2 received oleic acid which stimulates lipid droplet formation. Wells A3 – A6 received oleic acid plus increasing amounts of triacsin C, an inhibitor of lipid droplet formation (see Figure 7 for summary data).
| Cell by cell readout: | |||||||
|---|---|---|---|---|---|---|---|
| Well | Cell ID | LDC | Area Lm | Ratio (Lm/Wm) |
Tii Li Lm | Api Li Lm | Mpi Li Lm |
| A1 | 1 | 2 | 22 | 0.0168 | 1093 | 49.7 | 46 |
| A1 | 2 | 1 | 6 | 0.0036 | 207 | 34.5 | 35 |
| A1 | 3 | 0 | 0 | 0.0000 | 0 | 0.0 | 0 |
| A1 | 4 | 0 | 0 | 0.0000 | 0 | 0.0 | 0 |
| A1 | 5 | 1 | 14 | 0.0086 | 489 | 34.9 | 35 |
| A1 | 6 | 0 | 3 | 0.0012 | 70 | 23.3 | 22 |
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| Well by well readout (mean values): | |||||||
|---|---|---|---|---|---|---|---|
| Well | NOC | LDC | Area Lm | Ratio (Lm/Wm) |
Tii Li Lm | Api Li Lm | M pi Li Lm |
| A1 | 914 | 1.12 | 12.5 | 0.010 | 530 | 32.0 | 31.2 |
| A2 | 842 | 4.62 | 304.1 | 0.221 | 15005 | 48.4 | 45.8 |
| A3 | 848 | 4.43 | 296.1 | 0.212 | 14322 | 47.4 | 44.8 |
| A4 | 958 | 4.32 | 263.2 | 0.209 | 13520 | 50.4 | 47.4 |
| A5 | 991 | 4.23 | 261.0 | 0.213 | 13277 | 50.0 | 47.1 |
| A6 | 1005 | 3.96 | 237.6 | 0.193 | 11478 | 47.6 | 45.1 |
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