Table 3. Pr Li Pi Wm for human adipocytes as quantified by CyteSeer®.
Exp. 1 | PPARγ agonist (µM) | |||
---|---|---|---|---|
Protein | 0.1 | 1 | 10 | Mean ± SD |
HSL | 0.0978 | −0.0578 | −0.0431 | −0.00103 ± 0.0859* (n=3) |
PKC | 0.156 | 0.156 | 0.167 | 0.160 ± 0.00635* (n=3) |
Perilipin | 0.368 | 0.425 | 0.429 | 0.377 ± 0.0483* (n=5) |
0.322 | 0.342 |
Exp. 2 | Rosi(1 µM) |
---|---|
Perilipin | 0.480 ± 0.0691 (n=5) |
HCS CellMask™ | 0.186 ± 0.0437** (n=5) |
p < 0.05 vs. all other groups in Exp. 1 (ANOVA followed by Neuman Keuls post hoc analysis). For Exp. 2, cells were exposed to 1 µM rosi for 17 days, then fixed and stained for lipids and labeled for either perilipin or HCS CellMask™; data points are the average ± SD for n= 5 wells/condition (150 to 234 cells/well).
p <.01, vs. perilipin, (Student’s t-test).