Table 3. Pr Li Pi Wm for human adipocytes as quantified by CyteSeer®.
For Exp. 1,each data point represents a single well (73 to 252 cells/well). Since the Pr Li Pi Wm values for each protein were relatively constant across all agonist concentrations, the data points obtained for each well were averaged for comparison (final column).
Exp. 1 | PPARγ agonist (µM) | |||
---|---|---|---|---|
Protein | 0.1 | 1 | 10 | Mean ± SD |
HSL | 0.0978 | −0.0578 | −0.0431 | −0.00103 ± 0.0859* (n=3) |
PKC | 0.156 | 0.156 | 0.167 | 0.160 ± 0.00635* (n=3) |
Perilipin | 0.368 | 0.425 | 0.429 | 0.377 ± 0.0483* (n=5) |
0.322 | 0.342 |
Exp. 2 | Rosi(1 µM) |
---|---|
Perilipin | 0.480 ± 0.0691 (n=5) |
HCS CellMask™ | 0.186 ± 0.0437** (n=5) |
p < 0.05 vs. all other groups in Exp. 1 (ANOVA followed by Neuman Keuls post hoc analysis). For Exp. 2, cells were exposed to 1 µM rosi for 17 days, then fixed and stained for lipids and labeled for either perilipin or HCS CellMask™; data points are the average ± SD for n= 5 wells/condition (150 to 234 cells/well).
p <.01, vs. perilipin, (Student’s t-test).