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Primer and Probe Sets for RB1 and GJB2 Target Regions
| Forward primer | Reverse primer | Methylated probe | Unmethylated probe | |
|---|---|---|---|---|
| RB1 | 5’-GTTTAAGGAGGGAGAGTGG-3’ | 5’-AAATAACTATAAA CCTCATCCCTATCC-3’ | FAM5’-AACACGTCCGAACCGCGCCGAATAC3’-TAMRA | VIC5’-AACACATCCAAACCACACCAAATAC3’-TAMRA |
| GJB2 | 5’-GGAATTGATTTTTATTTTTTGGAG-3’ | 5’-AAAAAAACCACTAAAATCTTAACCC-3’ | FAM-5’CCGAAATCGACTAACTCCGCGTTA3’-TAMRA | VIC-5’CCAAAATCAACTAACTCCACATTA3’-TAMRA |
