Skip to main content
. Author manuscript; available in PMC: 2023 Dec 8.
Published in final edited form as: Cancer Immunol Immunother. 2023 Oct 17;72(12):3985–3999. doi: 10.1007/s00262-023-03530-3

Figure 1.

Figure 1.

Regulation of PD-L1 by NR4A1/Sp1 in human colon cancer cells. A. Model for regulation of PD-L1 and other genes with GC-rich promoters by NR4A1/Sp1 and structure of 3,5-disubstitutedphenyl DIM analogs. SW480 and RKO cells were transfected with oligonucleotides targeting NR4A1 (B), PD-L1 (C) and Sp1 (D) or treated with mithramycin (E) and whole cell lysates were analyzed by western blots as outlined in the Methods. SW480 and RKO cells (F) were treated with DIM-3-Br-5-OCF3 (X=Br, Y=OCF3, R=H) or DIM-3,5-Cl2 (X=Y=Cl; R=H) for 24 h and whole cell lysates were analyzed by western blot.