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Kinetic parameters for TEV redesigns and parent TEV variant.
| Variant |
kcat (min−1) |
Km (μM) |
kcat/Km (μM−1 min−1) |
Fold-improvement in kcat/Km over parent |
|---|---|---|---|---|
| hyperTEV56 | 0.0106 ± 0.0005 | 1.4 ± 0.2 | 0.0077 | 20 |
| hyperTEV60 | 0.014 ± 0.002 | 1.4 ± 0.4 | 0.01 | 26 |
| hyperTEV89 | 0.0050 ± 0.0001 | 2 ± 1 | 0.0024 | 6.2 |
| TEVd | 0.0023 ± 0.0003 | 6 ± 3 | 0.00039 |
