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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Neuropsychopharmacology. 2010 May 12;35(9):1962–1972. doi: 10.1038/npp.2010.70

Figure 6. The effect of ethanol on GABA transmission is reversed by WIN2.

Figure 6

A. Superfusion of ethanol (EtOH) onto this representative neuron increased the amplitude of the evoked IPSP. Addition of WIN2 in the continued presence of ethanol completely blocked the effect. RMP was -77 mV. B. Pooled data showing the reversal of the ethanol effect by WIN2. Ethanol (44 mM, t = 0) increased IPSP amplitudes to 141% of control. Subsequent addition of 2 μM WIN2 (t = 12) brought IPSP amplitudes back to 98% of pre-ethanol values. C. Top: recordings obtained using the paired-pulse paradigm. Ethanol increased the primary IPSP but slightly decreased the secondary IPSP, revealing a decrease of PPF, an effect completely reversed by WIN2. Bottom left: for scaled P1 amplitudes, P2 was much smaller in the presence of ethanol, an effect reversed by subsequent addition of WIN2. Bottom right: ethanol (44 mM) decreased the mean paired-pulse ratio to 68% of control, and 2 μM WIN2 brought PPF values back to 97% of pre-ethanol level. The data suggest that ethanol and WIN2 interact at a presynaptic site.