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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Neurosci. 2010 Apr 14;30(15):5334–5345. doi: 10.1523/JNEUROSCI.5963-09.2010

Figure 6.

Figure 6

Cell-autonomous role for Dlx5/6 in controlling differentiation of PV+ cortical interneurons. E13.5 Dlx5-/- or Dlx5/6-/- mutant MGE cells were transplanted into a wild type postnatal day 0 (P0) cortex; the Lhx6-BAC GFP transgene was a reporter to follow the fate of MGE-derived cells several weeks after transplantation. (A∼D) Neocortical interneurons differentiated from transplanted Lhx6-GFP-expressing Dlx5/6-/- precursors, as shown by double immunofluorescence with anti-PV, anti-SST, anti-NPY or anti-CR antibodies. E. Percentage of double-labeled cells in the neocortex of 2-month-old mice grafted with control (blue), Dlx5-/- (yellow) and Dlx5/6-/- (red) cells. The percentage of GFP+/PV+ double-positive cells is reduced in mice grafted with either the Dlx5-/- or Dlx5/6-/- MGE cells. To analyze the morphology of GFP+/PV+ interneurons, Z-stack confocal image for dendrite analysis was used; a representative GFP+/PV+ grafted cell from Dlx5/6-/- mutant is shown(F); the same cell was captured with Z-stack confocal image for dendrite analysis (F′). (G) Representative images of grafted PV+ neocortical interneurons from control, Dlx5-/- and Dlx5/6-/- mutants. (H∼K) Quantification of dendrite branching of PV+ interneurons from control (blue), Dlx5-/- (yellow) and Dlx5/6-/- (red) mutants. Note that, the number of branches is increased in Dlx5/6-/- grafted PV+ interneurons. Scale bar: 100μm.