Fig. 2. IFN-ϒ production in T cells and T conventional /T regulatory cells treated with CTLA-4 inhibitors.

a Mouse IFN-ϒ. Tumor-infiltrating lymphocytes from C57BL/6 mice and MC38 cells were cocultured with the top 5 compounds at 2 doses (10 μM, 50 μM). The IFN- ϒ value was measured with AlphaLISA. b Human IFN-ϒ stimulation. Dendritic cells and allogenic T-cells from human peripheral blood mononuclear cells were cocultured with the compounds at 2 doses (10 μM and 50 μM), and IFN-ϒ–positive cells were detected with flow cytometry. c The T_Conv:T_reg ratio in representative populations. The T_Conv:T_reg ratio was also determined with flow cytometry. d T_Conv and T_reg individual percentages and ratios. The table summarizes the percentages of T_conv and T_reg cells in cocultured dendritic cells and peripheral blood mononuclear cells treated with A9 and D11 (10 μM and 50 μM) compared to controls (n = 3). AlphaLISA amplified luminescent proximity homogeneous LISA, CTLA-4 cytotoxic T-lymphocyte–associated protein 4, Foxp3 forkhead box p3, IFN-γ interferon γ, T_conv T conventional, T_reg, T regulatory.