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. Author manuscript; available in PMC: 2024 Nov 13.
Published in final edited form as: Nat Biotechnol. 2024 Jan 11;42(11):1684–1692. doi: 10.1038/s41587-023-02085-z

Fig. 5. Functional dynamics of cellular engineering in vivo.

Fig. 5.

a, Depletion of CD19+ B cells is observed post-administration of T cell-targeted lentivirus (experiment 2). Human CD45+ cells were isolated from PBMC-humanized spleens 10 days post systemic administration of T cell-targeted Cas9-EDV (N=8 animals),lentivirus (N=8 animals), or PBS (N=4 animals), and the percentage of CD19-expressing cells was assessed by flow cytometry. P values calculated by means of Dunnett’s multiple comparison test after ordinary one-way ANOVA. **P < 0.01. Black lines indicate the median of the data set. PBS = phosphate buffered saline. b, Model for the in vivo generation of CAR T cells, with or without simultaneous genome editing. Schematic made with Biorender and is not to scale. c, T cell receptor clonotypes of CAR-transduced T cells isolated from humanized mice treated with T cell-targeted Cas9-EDV (mouse numbers 2, 4, 6) or lentivirus (mouse numbers 13, 14, 17) from experiment 2. “n” indicates the unique number of clonotypes detected.