Figure 6.

Analysis of short- and long-term memory in svr and transgenic CPD mutants, using an assay based on courtship behavior. The percentage of time a male spends engaged in courtship during a test period of ten minutes is referred to as the courtship index (CI) [44]. A. Wild type, svr^poi, and svr¹ mutants 0 minute courtship levels and 60 minute courtship levels are significantly suppressed (** indicates p<0.001*** indicates p<0.0005) compared to naïve courtship levels. Courtship levels are compared within genotype; the similar changes indicate that immediate recall memory and short-term memory at 0 and 60 minutes remain intact in the mutant flies. For naïve courtship, wild type flies n=16, svr^poi n=19, svr¹ n=18; 0 minute memory wild type flies n=19, svr^poi n=19, svr¹ n=20; for 60 minute memory wild type flies n=19, svr^poi n=20, svr¹ n=20. B. Comparison of long-term memory (LTM)-trained and naïve -trained courtship activity. Wild type flies show a reduction in courtship index in the LTM-trained group, relative to the Naïve-Trained group, indicating long-term memory. In contrast, the courtship index of the svr^poi and svr¹ flies is comparable between the LTM-trained and Naïve-trained groups, indicating no long-term memory. The LTM Trained courtship levels for the wild type, 2-3-t2, and 1B-short groups of flies are significantly suppressed compared to Naïve Trained courtship levels (* indicates p <0.05), indicating that LTM remains intact for 2-3-t2 and 1B-short transgenic CPD mutants. Wild type flies n=37 for naïve and LTM trained; svr^poi n=26 and 23; svr¹ n=26 and 20; 2-3-t2 n=19 and 16 respectively, and for 1B-short flies n=17.